Molecular genetic determination of human leukocyte antigens (HLA) using Luminex technology is based on a sequence-specific oligonucleotide (SSO) approach. In this method, HLA-specific probes are attached to microspheres, or beads. HLA PCR products bind to these probes only if they perfectly match the probe sequence. Each bead population carries only one specific probe. In a single assay, up to 100 different bead populations and thus sequence-specific probes can be used. For higher resolution assays, LABScan3D technology can accommodate up to 500 different bead populations.
The bead populations are quantified using two fluorescent dyes at varying concentrations, which are analyzed by the Luminex flow cytometer. One laser detects the fluorescence on the bead surface to distinguish bead populations, while a second laser measures fluorescence emitted by the labeled HLA PCR products bound to the probes. The final HLA typing result is determined by assessing the positive and negative binding reactions between the PCR products and the probes.
WAYS TO PARTNER WITH US
TarCET IVD Kits
CE-IVD kits containing reagents
for hereditary indications in
an easy-to-use kit form
Technology Transfer
Out-of-the-box genetics workflow
from sequencing to reporting
for non-invasive prenatal testing
Contact Us
Please get in touch with us
for any questions, inquiries,
feedback, or with any
comments you might have.
BLOG ARTICLES
Cystic fibrosis (CF) is a life-threatening, progressive, inherited condition that causes severe damage to the body, primarily affecting the organs of…
Down syndrome is a genetic condition caused by the presence of an extra copy of chromosome 21. It affects physical growth, facial features, and cogni…
Every year on April 25th, DNA Day celebrates the discovery of DNA’s double helix and the advances we’ve made in understanding genetics. D…
Neurodevelopmental disorders (NDDs) have diverse genetic origins, making diagnosis challenging. A new study analyzing over 1,100 pediatric patients f…
Colorectal cancer (CRC) remains one of the most prevalent and deadly cancers worldwide, with a significant number of cases presenting at an advanced …
Carrier screening is a genetic test designed to identify whether an individual carries a gene with changes (mutations) associated with inherited diso…
Trisomy 13, also known as Patau syndrome, is a genetic condition in which cells in the body have three copies of chromosome 13 instead of two. The co…
The human brain develops through a complex series of events, with genes carefully regulating the formation of neurons and glial cells. A recent study…
Rare diseases affect between 300 and 400 million people worldwide – more than cancer and AIDS combined [1, 2], but despite this, many people face a…
Rosalind Elsie Franklin was a brilliant and meticulous research scientist whose interdisciplinary work made fundamental contributions to various area…
